Coding
agaA
Part:BBa_J26006:Design
Designed by: Patrick King Group: iGEM06_Calgary (2006-07-27)
Beta Agarase A (raw DNA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 819
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1048
Illegal NgoMIV site found at 1120
Illegal AgeI site found at 730
Illegal AgeI site found at 952 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 733
Design Notes
This sequence hasn't been adapted as a biobrick yet, needs to be edited for that.
Source
Downloaded from http://getentry.ddbj.nig.ac.jp/cgi-bin/get_entry.pl?D21202 on 27-7-2006
Source paper: Sugano,Y., Matsumoto,T., Kodama,H. and Noma,M. , Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107 Appl. Environ. Microbiol. 59, 3750-3756 (1993)